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psrc y416  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc psrc y416
    Western blot analysis of the yield of phosphorylated ezrin-radixin-moesin (ERM) proteins after depletion of PDPN ( A ) or SRC ( B ) in BxPC-3 cells. The quantification of band intensities through densitometry is presented in supplementary . siNEG—control cells; siPDPN—cells with depleted podoplanin; pERM—phosphorylated ERM proteins; tERM—total ERM proteins; <t>pSRC—phosphorylated</t> SRC; tSRC—total SRC; β -actin—loading control.
    Psrc Y416, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/psrc+y416/pmc12523579-4-0-12
    Average 86 stars, based on 1 article reviews
    psrc y416 - by Bioz Stars, 2026-09
    86/100 stars

    Images

    1) Product Images from "Analysis of the Role of the SRC Tyrosine Kinase and Podoplanin in the Process of Entosis"

    Article Title: Analysis of the Role of the SRC Tyrosine Kinase and Podoplanin in the Process of Entosis

    Journal: Cancers

    doi: 10.3390/cancers17193173

    Western blot analysis of the yield of phosphorylated ezrin-radixin-moesin (ERM) proteins after depletion of PDPN ( A ) or SRC ( B ) in BxPC-3 cells. The quantification of band intensities through densitometry is presented in supplementary . siNEG—control cells; siPDPN—cells with depleted podoplanin; pERM—phosphorylated ERM proteins; tERM—total ERM proteins; pSRC—phosphorylated SRC; tSRC—total SRC; β -actin—loading control.
    Figure Legend Snippet: Western blot analysis of the yield of phosphorylated ezrin-radixin-moesin (ERM) proteins after depletion of PDPN ( A ) or SRC ( B ) in BxPC-3 cells. The quantification of band intensities through densitometry is presented in supplementary . siNEG—control cells; siPDPN—cells with depleted podoplanin; pERM—phosphorylated ERM proteins; tERM—total ERM proteins; pSRC—phosphorylated SRC; tSRC—total SRC; β -actin—loading control.

    Techniques Used: Western Blot, Control

    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1
    Article Snippet: Protein concentrations were determined by bicinchoninic acid protein assay (Pierce, Rockford, IL, USA). .. In all assays, 20–30 μg of protein was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (NuPAGE, Life Technologies, Carlsbad, CA, USA) and transferred by electrophoresis to a polyvinylidene difluoride (PVDF) membrane and developed with following antibodies; EpCAM (SCBT,sc-25308), Actin-HRP (SCBT, sc-47778), p-ERK1-T202,Y204 (Cell Signaling #4370), ERK1/2, (Cell Signaling #7102), pJNK-T183,Y185 (Cell Signaling #4668), JNK, (Cell Signaling #9258), pP38-T180/Y182 (Cell Signaling #4511), P38 (Cell Signaling #9212), pATK-S473 (Cell Signaling #4051), AKT, (Cell Signaling #9212), pP65-S536 (Cell Signaling #3033), P65 (Cell Signaling #8242), pSrc-Y416 (Cell Signaling #2101), SRC, (Cell Signaling #2108), Cleaved PARP, (Cell Signaling #5625), Cleaved Caspase-3 (Cell Signaling #9661), Pan-Ras (SCBT, sc-166691), H-Ras (Cell Signaling #3339), E-cadherin (SCBT, Sc-7870), ZEB1 (SCBT, Sc-25388), FRA1 (Cell Signaling #5281), Vimentin (Cell Signaling #3516), Snail (Cell Signaling #3879), CTNNB1 Cell Signaling #8480). .. Signal detection was performed using the Super-Signal West Pico chemiluminescent immunodetection system (Thermo Scientific, Rockford, IL, USA).

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: Protein concentrations were determined by bicinchoninic acid protein assay (Pierce, Rockford, IL, USA). .. In all assays, 20–30 μg of protein was subjected to sodium dodecyl sulfatepolyacrylamide gel electrophoresis (NuPAGE, Life Technologies, Carlsbad, CA, USA) and transferred by electrophoresis to a polyvinylidene difluoride (PVDF) membrane and developed with following antibodies; EpCAM (SCBT,sc-25308), Actin-HRP (SCBT ,sc-47778), pERK1-T202,Y204 (Cell Signaling #4370), ERK1/2, (Cell Signaling #7102), pJNK-T183,Y185 (Cell Signaling #4668), JNK, (Cell Signaling #9258), pP38-T180/Y182 (Cell Signaling #4511), P38 (Cell Signaling #9212), pATK-S473 (Cell Signaling #4051), AKT, (Cell Signaling #9212), pP65-S536 (Cell Signaling #3033), P65 (Cell Signaling #8242), pSrc-Y416 (Cell Signaling #2101), SRC, (Cell Signaling #2108), Cleaved PARP, (Cell Signaling #5625), Cleaved Caspase-3 (Cell Signaling #9661), Pan-Ras (SCBT, sc-166691), H-Ras (Cell Signaling #3339), E-cadherin (SCBT, Sc-7870), ZEB1 (SCBT, Sc-25388), FRA1 (Cell Signaling #5281), Vimentin (Cell Signaling #3516), Snail (Cell Signaling #3879), CTNNB1 Cell Signaling #8480). .. Signal detection was performed using the Super-Signal West Pico chemiluminescent immunodetection system (Thermo Scientific, Rockford, IL, USA).

    Electrophoresis:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1
    Article Snippet: Protein concentrations were determined by bicinchoninic acid protein assay (Pierce, Rockford, IL, USA). .. In all assays, 20–30 μg of protein was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (NuPAGE, Life Technologies, Carlsbad, CA, USA) and transferred by electrophoresis to a polyvinylidene difluoride (PVDF) membrane and developed with following antibodies; EpCAM (SCBT,sc-25308), Actin-HRP (SCBT, sc-47778), p-ERK1-T202,Y204 (Cell Signaling #4370), ERK1/2, (Cell Signaling #7102), pJNK-T183,Y185 (Cell Signaling #4668), JNK, (Cell Signaling #9258), pP38-T180/Y182 (Cell Signaling #4511), P38 (Cell Signaling #9212), pATK-S473 (Cell Signaling #4051), AKT, (Cell Signaling #9212), pP65-S536 (Cell Signaling #3033), P65 (Cell Signaling #8242), pSrc-Y416 (Cell Signaling #2101), SRC, (Cell Signaling #2108), Cleaved PARP, (Cell Signaling #5625), Cleaved Caspase-3 (Cell Signaling #9661), Pan-Ras (SCBT, sc-166691), H-Ras (Cell Signaling #3339), E-cadherin (SCBT, Sc-7870), ZEB1 (SCBT, Sc-25388), FRA1 (Cell Signaling #5281), Vimentin (Cell Signaling #3516), Snail (Cell Signaling #3879), CTNNB1 Cell Signaling #8480). .. Signal detection was performed using the Super-Signal West Pico chemiluminescent immunodetection system (Thermo Scientific, Rockford, IL, USA).

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: Protein concentrations were determined by bicinchoninic acid protein assay (Pierce, Rockford, IL, USA). .. In all assays, 20–30 μg of protein was subjected to sodium dodecyl sulfatepolyacrylamide gel electrophoresis (NuPAGE, Life Technologies, Carlsbad, CA, USA) and transferred by electrophoresis to a polyvinylidene difluoride (PVDF) membrane and developed with following antibodies; EpCAM (SCBT,sc-25308), Actin-HRP (SCBT ,sc-47778), pERK1-T202,Y204 (Cell Signaling #4370), ERK1/2, (Cell Signaling #7102), pJNK-T183,Y185 (Cell Signaling #4668), JNK, (Cell Signaling #9258), pP38-T180/Y182 (Cell Signaling #4511), P38 (Cell Signaling #9212), pATK-S473 (Cell Signaling #4051), AKT, (Cell Signaling #9212), pP65-S536 (Cell Signaling #3033), P65 (Cell Signaling #8242), pSrc-Y416 (Cell Signaling #2101), SRC, (Cell Signaling #2108), Cleaved PARP, (Cell Signaling #5625), Cleaved Caspase-3 (Cell Signaling #9661), Pan-Ras (SCBT, sc-166691), H-Ras (Cell Signaling #3339), E-cadherin (SCBT, Sc-7870), ZEB1 (SCBT, Sc-25388), FRA1 (Cell Signaling #5281), Vimentin (Cell Signaling #3516), Snail (Cell Signaling #3879), CTNNB1 Cell Signaling #8480). .. Signal detection was performed using the Super-Signal West Pico chemiluminescent immunodetection system (Thermo Scientific, Rockford, IL, USA).

    Membrane:

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1
    Article Snippet: Protein concentrations were determined by bicinchoninic acid protein assay (Pierce, Rockford, IL, USA). .. In all assays, 20–30 μg of protein was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (NuPAGE, Life Technologies, Carlsbad, CA, USA) and transferred by electrophoresis to a polyvinylidene difluoride (PVDF) membrane and developed with following antibodies; EpCAM (SCBT,sc-25308), Actin-HRP (SCBT, sc-47778), p-ERK1-T202,Y204 (Cell Signaling #4370), ERK1/2, (Cell Signaling #7102), pJNK-T183,Y185 (Cell Signaling #4668), JNK, (Cell Signaling #9258), pP38-T180/Y182 (Cell Signaling #4511), P38 (Cell Signaling #9212), pATK-S473 (Cell Signaling #4051), AKT, (Cell Signaling #9212), pP65-S536 (Cell Signaling #3033), P65 (Cell Signaling #8242), pSrc-Y416 (Cell Signaling #2101), SRC, (Cell Signaling #2108), Cleaved PARP, (Cell Signaling #5625), Cleaved Caspase-3 (Cell Signaling #9661), Pan-Ras (SCBT, sc-166691), H-Ras (Cell Signaling #3339), E-cadherin (SCBT, Sc-7870), ZEB1 (SCBT, Sc-25388), FRA1 (Cell Signaling #5281), Vimentin (Cell Signaling #3516), Snail (Cell Signaling #3879), CTNNB1 Cell Signaling #8480). .. Signal detection was performed using the Super-Signal West Pico chemiluminescent immunodetection system (Thermo Scientific, Rockford, IL, USA).

    Article Title: Cytosolic EpCAM cooperates with H-Ras to regulate epithelial to mesenchymal transition through ZEB1.
    Article Snippet: Protein concentrations were determined by bicinchoninic acid protein assay (Pierce, Rockford, IL, USA). .. In all assays, 20–30 μg of protein was subjected to sodium dodecyl sulfatepolyacrylamide gel electrophoresis (NuPAGE, Life Technologies, Carlsbad, CA, USA) and transferred by electrophoresis to a polyvinylidene difluoride (PVDF) membrane and developed with following antibodies; EpCAM (SCBT,sc-25308), Actin-HRP (SCBT ,sc-47778), pERK1-T202,Y204 (Cell Signaling #4370), ERK1/2, (Cell Signaling #7102), pJNK-T183,Y185 (Cell Signaling #4668), JNK, (Cell Signaling #9258), pP38-T180/Y182 (Cell Signaling #4511), P38 (Cell Signaling #9212), pATK-S473 (Cell Signaling #4051), AKT, (Cell Signaling #9212), pP65-S536 (Cell Signaling #3033), P65 (Cell Signaling #8242), pSrc-Y416 (Cell Signaling #2101), SRC, (Cell Signaling #2108), Cleaved PARP, (Cell Signaling #5625), Cleaved Caspase-3 (Cell Signaling #9661), Pan-Ras (SCBT, sc-166691), H-Ras (Cell Signaling #3339), E-cadherin (SCBT, Sc-7870), ZEB1 (SCBT, Sc-25388), FRA1 (Cell Signaling #5281), Vimentin (Cell Signaling #3516), Snail (Cell Signaling #3879), CTNNB1 Cell Signaling #8480). .. Signal detection was performed using the Super-Signal West Pico chemiluminescent immunodetection system (Thermo Scientific, Rockford, IL, USA).

    other:

    Article Title: RANK-C Expression Sensitizes ER-Negative, EGFR-Positive Breast Cancer Cells to EGFR-Tyrosine Kinase Inhibitors (TKIs)
    Article Snippet: The antibodies employed in this study were: EGFR (E235, 04-338; 1:4000 Millipore, MA, USA), pEGFR-Y845 (AF-3394; 1:100, R&D systems), pEGFR-Y1173 (04-341; 1:1000, Millipore), pAKT-S473 (4060; 1:2000, CST), ERK1/2 (9102; 1:1000, CST), pERK1/2-T202/Y204 (4376; 1:1000, CST), pSRC-Y416 (2101; 1:1000, CST).

    Western Blot:

    Article Title: Fluid shear stress facilitates prostate cancer metastasis through Piezo1-Src-YAP axis
    Article Snippet: .. Immunoblotting was performed using standard procedures with pYAP(S127) (Cell Signaling Technology, Danvers, Massachusetts, USA), YAP (Y357) (Cell Signaling Technology), YAP (Cell Signaling Technology), TAZ (Thermo Fisher Scienti c), pSrc(Y416) (Cell Signaling Technology), Src (Cell Signaling Technology), and GAPDH (Santa Cruz Biotechnology, Dallas, TX, USA). .. Gel images were captured using LAS4000 (GE Healthcare Life Sciences, Chicago, IL, USA).

    Aqueous Normal-phase Chromatography:

    Article Title: Cx45 regulation by kinases and impact of expression in heart failure.
    Article Snippet: Phosphorylation plays a crucial role in connexin regulation by modulating gap junction intercellular communication (GJIC), localization, stability, and interactions with signaling proteins.. Few kinases are known to phosphorylate Cx45, and their target residues remain unknown.. A phosphorylation screen identified several Cx45targeting kinases activated in heart disease, among which c-Src was found by mass spectroscopy to phosphorylate residues Y324 and Y356.



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    Western blot analysis of the yield of phosphorylated ezrin-radixin-moesin (ERM) proteins after depletion of PDPN ( A ) or SRC ( B ) in BxPC-3 cells. The quantification of band intensities through densitometry is presented in supplementary . siNEG—control cells; siPDPN—cells with depleted podoplanin; pERM—phosphorylated ERM proteins; tERM—total ERM proteins; <t>pSRC—phosphorylated</t> SRC; tSRC—total SRC; β -actin—loading control.
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    Image Search Results


    Western blot analysis of the yield of phosphorylated ezrin-radixin-moesin (ERM) proteins after depletion of PDPN ( A ) or SRC ( B ) in BxPC-3 cells. The quantification of band intensities through densitometry is presented in supplementary . siNEG—control cells; siPDPN—cells with depleted podoplanin; pERM—phosphorylated ERM proteins; tERM—total ERM proteins; pSRC—phosphorylated SRC; tSRC—total SRC; β -actin—loading control.

    Journal: Cancers

    Article Title: Analysis of the Role of the SRC Tyrosine Kinase and Podoplanin in the Process of Entosis

    doi: 10.3390/cancers17193173

    Figure Lengend Snippet: Western blot analysis of the yield of phosphorylated ezrin-radixin-moesin (ERM) proteins after depletion of PDPN ( A ) or SRC ( B ) in BxPC-3 cells. The quantification of band intensities through densitometry is presented in supplementary . siNEG—control cells; siPDPN—cells with depleted podoplanin; pERM—phosphorylated ERM proteins; tERM—total ERM proteins; pSRC—phosphorylated SRC; tSRC—total SRC; β -actin—loading control.

    Article Snippet: pSRC (Y416) , 2101 , Rabbit polyclonal , 1:1000/ 5% BSA , Cell Signaling Technology, Inc..

    Techniques: Western Blot, Control